Talaromyces thermophilus beta-D-xylosidase: purification, characterization and xylobiose synthesis.
Identifieur interne : 000726 ( Main/Exploration ); précédent : 000725; suivant : 000727Talaromyces thermophilus beta-D-xylosidase: purification, characterization and xylobiose synthesis.
Auteurs : Mohamed Guerfali [Tunisie] ; Ali Gargouri ; Hafedh BelghithSource :
- Applied biochemistry and biotechnology [ 0273-2289 ] ; 2008.
Descripteurs français
- KwdFr :
- Activation enzymatique (), Calcium (pharmacologie), Chromatographie, Chromatographie en phase liquide à haute performance, Chromatographie sur gel, Cinétique, Cobalt (pharmacologie), Concentration en ions d'hydrogène, Cuivre (pharmacologie), Diholoside (biosynthèse), Manganèse (pharmacologie), Masse moléculaire, Mercure (pharmacologie), Protéines fongiques (), Protéines fongiques (isolement et purification), Protéines fongiques (métabolisme), Talaromyces (), Talaromyces (enzymologie), Température, Xylosidases (), Xylosidases (isolement et purification), Xylosidases (métabolisme), Zinc (pharmacologie), Électrophorèse sur gel de polyacrylamide.
- MESH :
- biosynthèse : Diholoside.
- enzymologie : Talaromyces.
- isolement et purification : Protéines fongiques, Xylosidases.
- métabolisme : Protéines fongiques, Xylosidases.
- pharmacologie : Calcium, Cobalt, Cuivre, Manganèse, Mercure, Zinc.
- Activation enzymatique, Chromatographie, Chromatographie en phase liquide à haute performance, Chromatographie sur gel, Cinétique, Concentration en ions d'hydrogène, Masse moléculaire, Protéines fongiques, Talaromyces, Température, Xylosidases, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Calcium (pharmacology), Chromatography, Chromatography, Gel, Chromatography, High Pressure Liquid, Cobalt (pharmacology), Copper (pharmacology), Disaccharides (biosynthesis), Electrophoresis, Polyacrylamide Gel, Enzyme Activation (drug effects), Fungal Proteins (chemistry), Fungal Proteins (isolation & purification), Fungal Proteins (metabolism), Hydrogen-Ion Concentration, Kinetics, Manganese (pharmacology), Mercury (pharmacology), Molecular Weight, Talaromyces (drug effects), Talaromyces (enzymology), Temperature, Xylosidases (chemistry), Xylosidases (isolation & purification), Xylosidases (metabolism), Zinc (pharmacology).
- MESH :
- chemical , biosynthesis : Disaccharides.
- chemical , chemistry : Fungal Proteins, Xylosidases.
- chemical , isolation & purification : Fungal Proteins, Xylosidases.
- chemical , metabolism : Fungal Proteins, Xylosidases.
- chemical , pharmacology : Calcium, Cobalt, Copper, Manganese, Mercury, Zinc.
- drug effects : Enzyme Activation, Talaromyces.
- enzymology : Talaromyces.
- Chromatography, Chromatography, Gel, Chromatography, High Pressure Liquid, Electrophoresis, Polyacrylamide Gel, Hydrogen-Ion Concentration, Kinetics, Molecular Weight, Temperature.
Abstract
When grown on wheat bran as the only carbon source, the filamentous fungus Talaromyces thermophilus produces large amounts of beta-xylosidase activity. The presence of glucose drastically decreases the beta-xylosidase production level. The beta-xylosidase of T. thermophilus was purified by ammonium sulfate precipitation, DEAE-cellulose chromatography, and gel filtration (high-performance liquid chromatography). The molecular mass of the enzyme was estimated to be 97 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis and gel filtration. The enzyme activity was optimum at 50 degrees C and pH 7. The apparent Michaelis constant K(m) of the beta-xylosidase was 2.37 mM for p-nitrophenyl-beta-D-xylopyranoside, with a V(max) of 0.049 micromol min(-1) per milligram protein. Enzyme activity was inhibited by Cu(2+), Hg(2+), and Zn(2+) and activated by Ca(2+), Mn(2+), and Co(+) at a concentration of 5 mM. At high xylose concentration, this enzyme catalyses the condensation reaction leading to xylobiose production.
DOI: 10.1007/s12010-008-8260-x
PubMed: 18592411
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000060
- to stream PubMed, to step Curation: 000060
- to stream PubMed, to step Checkpoint: 000060
- to stream Ncbi, to step Merge: 000082
- to stream Ncbi, to step Curation: 000082
- to stream Ncbi, to step Checkpoint: 000082
- to stream Main, to step Merge: 000732
- to stream Main, to step Curation: 000726
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Talaromyces thermophilus beta-D-xylosidase: purification, characterization and xylobiose synthesis.</title><author><name sortKey="Guerfali, Mohamed" sort="Guerfali, Mohamed" uniqKey="Guerfali M" first="Mohamed" last="Guerfali">Mohamed Guerfali</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes, Centre de Biotechnologie de Sfax, PB"K" 3038, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire de Génétique Moléculaire des Eucaryotes, Centre de Biotechnologie de Sfax, PB"K" 3038, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Gargouri, Ali" sort="Gargouri, Ali" uniqKey="Gargouri A" first="Ali" last="Gargouri">Ali Gargouri</name></author><author><name sortKey="Belghith, Hafedh" sort="Belghith, Hafedh" uniqKey="Belghith H" first="Hafedh" last="Belghith">Hafedh Belghith</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18592411</idno><idno type="pmid">18592411</idno><idno type="doi">10.1007/s12010-008-8260-x</idno><idno type="wicri:Area/PubMed/Corpus">000060</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000060</idno><idno type="wicri:Area/PubMed/Curation">000060</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000060</idno><idno type="wicri:Area/PubMed/Checkpoint">000060</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000060</idno><idno type="wicri:Area/Ncbi/Merge">000082</idno><idno type="wicri:Area/Ncbi/Curation">000082</idno><idno type="wicri:Area/Ncbi/Checkpoint">000082</idno><idno type="wicri:doubleKey">0273-2289:2008:Guerfali M:talaromyces:thermophilus:beta</idno><idno type="wicri:Area/Main/Merge">000732</idno><idno type="wicri:Area/Main/Curation">000726</idno><idno type="wicri:Area/Main/Exploration">000726</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Talaromyces thermophilus beta-D-xylosidase: purification, characterization and xylobiose synthesis.</title><author><name sortKey="Guerfali, Mohamed" sort="Guerfali, Mohamed" uniqKey="Guerfali M" first="Mohamed" last="Guerfali">Mohamed Guerfali</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Génétique Moléculaire des Eucaryotes, Centre de Biotechnologie de Sfax, PB"K" 3038, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratoire de Génétique Moléculaire des Eucaryotes, Centre de Biotechnologie de Sfax, PB"K" 3038, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Gargouri, Ali" sort="Gargouri, Ali" uniqKey="Gargouri A" first="Ali" last="Gargouri">Ali Gargouri</name></author><author><name sortKey="Belghith, Hafedh" sort="Belghith, Hafedh" uniqKey="Belghith H" first="Hafedh" last="Belghith">Hafedh Belghith</name></author></analytic><series><title level="j">Applied biochemistry and biotechnology</title><idno type="ISSN">0273-2289</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Calcium (pharmacology)</term><term>Chromatography</term><term>Chromatography, Gel</term><term>Chromatography, High Pressure Liquid</term><term>Cobalt (pharmacology)</term><term>Copper (pharmacology)</term><term>Disaccharides (biosynthesis)</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Enzyme Activation (drug effects)</term><term>Fungal Proteins (chemistry)</term><term>Fungal Proteins (isolation & purification)</term><term>Fungal Proteins (metabolism)</term><term>Hydrogen-Ion Concentration</term><term>Kinetics</term><term>Manganese (pharmacology)</term><term>Mercury (pharmacology)</term><term>Molecular Weight</term><term>Talaromyces (drug effects)</term><term>Talaromyces (enzymology)</term><term>Temperature</term><term>Xylosidases (chemistry)</term><term>Xylosidases (isolation & purification)</term><term>Xylosidases (metabolism)</term><term>Zinc (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation enzymatique ()</term><term>Calcium (pharmacologie)</term><term>Chromatographie</term><term>Chromatographie en phase liquide à haute performance</term><term>Chromatographie sur gel</term><term>Cinétique</term><term>Cobalt (pharmacologie)</term><term>Concentration en ions d'hydrogène</term><term>Cuivre (pharmacologie)</term><term>Diholoside (biosynthèse)</term><term>Manganèse (pharmacologie)</term><term>Masse moléculaire</term><term>Mercure (pharmacologie)</term><term>Protéines fongiques ()</term><term>Protéines fongiques (isolement et purification)</term><term>Protéines fongiques (métabolisme)</term><term>Talaromyces ()</term><term>Talaromyces (enzymologie)</term><term>Température</term><term>Xylosidases ()</term><term>Xylosidases (isolement et purification)</term><term>Xylosidases (métabolisme)</term><term>Zinc (pharmacologie)</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Disaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fungal Proteins</term><term>Xylosidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Fungal Proteins</term><term>Xylosidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term><term>Xylosidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Calcium</term><term>Cobalt</term><term>Copper</term><term>Manganese</term><term>Mercury</term><term>Zinc</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Diholoside</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Enzyme Activation</term><term>Talaromyces</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Talaromyces</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Talaromyces</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines fongiques</term><term>Xylosidases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines fongiques</term><term>Xylosidases</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Calcium</term><term>Cobalt</term><term>Cuivre</term><term>Manganèse</term><term>Mercure</term><term>Zinc</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography</term><term>Chromatography, Gel</term><term>Chromatography, High Pressure Liquid</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Hydrogen-Ion Concentration</term><term>Kinetics</term><term>Molecular Weight</term><term>Temperature</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation enzymatique</term><term>Chromatographie</term><term>Chromatographie en phase liquide à haute performance</term><term>Chromatographie sur gel</term><term>Cinétique</term><term>Concentration en ions d'hydrogène</term><term>Masse moléculaire</term><term>Protéines fongiques</term><term>Talaromyces</term><term>Température</term><term>Xylosidases</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">When grown on wheat bran as the only carbon source, the filamentous fungus Talaromyces thermophilus produces large amounts of beta-xylosidase activity. The presence of glucose drastically decreases the beta-xylosidase production level. The beta-xylosidase of T. thermophilus was purified by ammonium sulfate precipitation, DEAE-cellulose chromatography, and gel filtration (high-performance liquid chromatography). The molecular mass of the enzyme was estimated to be 97 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis and gel filtration. The enzyme activity was optimum at 50 degrees C and pH 7. The apparent Michaelis constant K(m) of the beta-xylosidase was 2.37 mM for p-nitrophenyl-beta-D-xylopyranoside, with a V(max) of 0.049 micromol min(-1) per milligram protein. Enzyme activity was inhibited by Cu(2+), Hg(2+), and Zn(2+) and activated by Ca(2+), Mn(2+), and Co(+) at a concentration of 5 mM. At high xylose concentration, this enzyme catalyses the condensation reaction leading to xylobiose production.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Belghith, Hafedh" sort="Belghith, Hafedh" uniqKey="Belghith H" first="Hafedh" last="Belghith">Hafedh Belghith</name><name sortKey="Gargouri, Ali" sort="Gargouri, Ali" uniqKey="Gargouri A" first="Ali" last="Gargouri">Ali Gargouri</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Guerfali, Mohamed" sort="Guerfali, Mohamed" uniqKey="Guerfali M" first="Mohamed" last="Guerfali">Mohamed Guerfali</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/CobaltMaghrebV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000726 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000726 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Terre
|area= CobaltMaghrebV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:18592411
|texte= Talaromyces thermophilus beta-D-xylosidase: purification, characterization and xylobiose synthesis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:18592411" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a CobaltMaghrebV1
| This area was generated with Dilib version V0.6.32. |  |